Cloning and sequencing of mouse GABA transporter complementary DNA
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چکیده
منابع مشابه
Molecular cloning and sequencing of DNA complementary to chicken liver fatty acid synthase mRNA.
The cDNA corresponding to 4.18 kilobases (kb) of the mRNA of chicken liver fatty acid synthase has been cloned and sequenced. The cDNA corresponds to the 3' end of the mRNA and consists of a 1.87-kb noncoding tail and a 2.31-kb region encoding 769 amino acids of the C terminus of the enzyme. The thioesterase at the C terminus, preceded by the acyl carrier protein, can be identified from known a...
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The cDNA encoding mouse ferrochelatase (protoheme ferrolyase, EC 4.99.1.1) was isolated from a mouse erythroleukemia (MEL) cell cDNA library in lambda gt11 expression vector, by immunoscreening with a polyclonal antibody. Two full-length clones containing cDNA inserts of 2.2 and 2.90 kilobases were obtained. These clones have the same entire enzyme coding region, but alternative putative polyad...
متن کاملHuman growth hormone: complementary DNA cloning and expression in bacteria.
The nucleotide sequence of a DNA complementary to human growth hormone messenger RNA was cloned; it contains 29 nucleotides in its 5' untranslated region, the 651 nucleotides coding for the prehormone, and the entire 3' untranslated region (108 nucleotides). The data reported predict the previously unknown sequence of the signal peptide of human growth hormone and, by comparison with the previo...
متن کاملCloning of DNA Complementary to Rat Liver NADPH-Cytochrome
NADPH-cytochrome c oxidoreductase and cytochrome P-450b mRNAs were enriched from tightly membrane-bound polysomes isolated from the livers of rats administered phenobarbital. This was accomplished by polysome immunoadsorption with monospecific I g G directed toward the oxidoreductase and cytochrome P-450b in conjunction with Staphylococcus aureus ghosts and oligo(dT)-cellulose chromatography. D...
متن کاملCloning, expression, and spectral analysis of mouse betatrophin
Background: Betatrophin, a novel secretory protein from liver and fatty tissues, is believed to be involved in lipid and glucose metabolism. However, its precise physiological role remains unclear. Here, we report the cloning, expression, and purification steps of mouse betatrophin in a prokaryotic system, followed by its structural analysis. Methods: Specific cloning primers were...
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ژورنال
عنوان ژورنال: Cell Research
سال: 1994
ISSN: 1001-0602,1748-7838
DOI: 10.1038/cr.1994.11